Extended sequence analysis of three Danish potato mop-top virus (PMTV) isolates
Language English Country United States Media print
Document type Comparative Study, Journal Article
PubMed
15284485
DOI
10.1023/b:viru.0000036385.87739.e4
PII: 5276719
Knihovny.cz E-resources
- MeSH
- Phylogeny MeSH
- Genome, Viral MeSH
- Molecular Sequence Data MeSH
- Plant Diseases virology MeSH
- RNA Viruses genetics isolation & purification MeSH
- Plant Viruses genetics isolation & purification MeSH
- Sequence Analysis, DNA * MeSH
- Solanum tuberosum virology MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Geographicals
- Denmark MeSH
The entire nucleotide sequence for the coding regions of a Danish PMTV isolate 54-15 was determined and compared to other known and sequenced isolates of PMTV. Many nucleotide and amino acid changes were found in parts of RNA coding for the triple gene block (TGB) proteins and in the part of the RNA coding for the read-through region of the coat protein (CP). These regions for two other isolates, the mild one 54-10 and the severe one 54-19, were sequenced. Only two amino acid changes were found to correlate with the subdivision of isolates according to symptom development into mild and severe subgroups. In addition, the phylogenetic tree was obtained suggesting the closest relationship between isolates 54-15 and 54-10. Although the sequence comparisons indicate a high genetic stability of PMTV populations, a surprising change was found in the newly sequenced isolates--the replacement of the AUG start codon of the fourth gene of the TGB encoding RNA, coding for a cystein-rich protein, by the less efficient GUG start codon.
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Short communication: Molecular analysis of Potato leafroll virus isolates from the Czech Republic
Sequence analysis of the Czech potato mop-top virus (PMTV) isolate Korneta-Nemilkov
GENBANK
AF487407, AF508253, AJ224991, AJ238607, AJ243719, AJ277556, AY187010, AY196094, AY196959, AY277633, AY353719, AY424977, AY424978, AY424979, AY426745, AY436586
