DNA microarray: parallel detection of potato viruses
Jazyk angličtina Země Švýcarsko Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
12828343
Knihovny.cz E-zdroje
- MeSH
- DNA primery genetika MeSH
- DNA virů analýza izolace a purifikace MeSH
- nemoci rostlin virologie MeSH
- Potexvirus genetika izolace a purifikace MeSH
- Potyvirus genetika izolace a purifikace MeSH
- rostlinné viry genetika izolace a purifikace MeSH
- sekvenční analýza DNA MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů metody statistika a číselné údaje MeSH
- sekvenční homologie MeSH
- senzitivita a specificita MeSH
- Solanum tuberosum virologie MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA primery MeSH
- DNA virů MeSH
DNA microarray assay has become a useful tool for gene expression studies. Less frequent is its application to detection of viruses or diagnostics of virus diseases. Here we show design of a microscope slide-based microarray assay for simultaneous identification of several potato viruses. Different primer pairs were designed or adopted to obtain specific amplicons from six potato viruses: Potato virus A (PVA), Potato virus S (PVS), Potato virus X (PVX), Potato virus Y (PVY), Potato mop-top virus (PMTV) and Potato leaf-roll virus (PLRV). Purified viral DNA probes were spotted on a microscope slide coated with poly-L-lysine. The same primers were used for preparation of fluorochrome-labeled targets. The latter were denatured and hybridized on the microarray slide (chip). An example of simultaneous assay of two pathogens is given and possibilities of practical application of this type of assay are discussed.
Extended sequence analysis of three Danish potato mop-top virus (PMTV) isolates
