DNA microarray: parallel detection of potato viruses
Language English Country Switzerland Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12828343
Knihovny.cz E-resources
- MeSH
- DNA Primers genetics MeSH
- DNA, Viral analysis isolation & purification MeSH
- Plant Diseases virology MeSH
- Potexvirus genetics isolation & purification MeSH
- Potyvirus genetics isolation & purification MeSH
- Plant Viruses genetics isolation & purification MeSH
- Sequence Analysis, DNA MeSH
- Oligonucleotide Array Sequence Analysis methods statistics & numerical data MeSH
- Sequence Homology MeSH
- Sensitivity and Specificity MeSH
- Solanum tuberosum virology MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Primers MeSH
- DNA, Viral MeSH
DNA microarray assay has become a useful tool for gene expression studies. Less frequent is its application to detection of viruses or diagnostics of virus diseases. Here we show design of a microscope slide-based microarray assay for simultaneous identification of several potato viruses. Different primer pairs were designed or adopted to obtain specific amplicons from six potato viruses: Potato virus A (PVA), Potato virus S (PVS), Potato virus X (PVX), Potato virus Y (PVY), Potato mop-top virus (PMTV) and Potato leaf-roll virus (PLRV). Purified viral DNA probes were spotted on a microscope slide coated with poly-L-lysine. The same primers were used for preparation of fluorochrome-labeled targets. The latter were denatured and hybridized on the microarray slide (chip). An example of simultaneous assay of two pathogens is given and possibilities of practical application of this type of assay are discussed.
Extended sequence analysis of three Danish potato mop-top virus (PMTV) isolates
