New performance of biosensor technology for Alzheimer's disease drugs: in vitro comparison of tacrine and 7-methoxytacrine
Jazyk angličtina Země Švédsko Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
18987590
PII: NEL290508A24
Knihovny.cz E-zdroje
- MeSH
- acetylcholinesterasa chemie MeSH
- algoritmy MeSH
- Alzheimerova nemoc farmakoterapie MeSH
- biosenzitivní techniky * MeSH
- cholinesterasové inhibitory analýza MeSH
- elektrochemie MeSH
- enzymy imobilizované chemie MeSH
- kinetika MeSH
- lidé MeSH
- nootropní látky analýza MeSH
- takrin analogy a deriváty analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- 7-methoxytacrine MeSH Prohlížeč
- acetylcholinesterasa MeSH
- cholinesterasové inhibitory MeSH
- enzymy imobilizované MeSH
- nootropní látky MeSH
- takrin MeSH
Two drugs were tested using electrochemical biosensor with immobilized acetylcholinesterase (AChE). The first was commercialized drug tacrine (known also as Cognex) used for treatment of cognitive manifestation of Alzheimer\'s disease (AD). The second one was its 7-methoxy derivate (7-MEOTA) that has not been marketed. We determined the IC50 (6.67+/-0.92)x10-7 M for tacrine and (1.66+/-1.43)x10-9 M for 7-MEOTA. In this in vitro study, 7-MEOTA acts as stronger inhibitor of AChE and in this way could be more favorable for treatment of cognitive manifestation of AD. Our study shows that biosensor technology could be used as a quick and cheap tool for testing of promising AChE inhibitors (AD drug candidates).
Cholinesterase based amperometric biosensors for assay of anticholinergic compounds
