Response to cadmium of Daucus carota hairy roots dual cultures with Glomus intraradices or Gigaspora margarita
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- houby fyziologie MeSH
- kadmium farmakologie MeSH
- kořeny rostlin účinky léků mikrobiologie fyziologie MeSH
- mrkev obecná účinky léků mikrobiologie fyziologie MeSH
- mykorhiza fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kadmium MeSH
Ri T-DNA-transformed carrot roots were cultivated in two experiments either non-inoculated or inoculated with the arbuscular mycorrhizal (AM) fungi Glomus intraradices or Gigaspora margarita. The influence of two concentrations of cadmium (Cd) in the medium (2 mg l(-1), 4 mg l(-1)) on both root and mycelium growth was tested. Both parameters were estimated at 10-day intervals for 70 or 100 days for G. intraradices and Gi. margarita, respectively. In the first experiment, G. intraradices showed a rapid spread of extraradical mycelium (ERM) and reached average densities per treatment of about 90 cm cm(-2) agar medium after 70 days. At the higher Cd level, the growth of ERM was delayed in comparison to the treatment without Cd addition. Root growth was inhibited by both Cd levels; the inhibition was, however, significantly lower in the treatments inoculated with G. intraradices compared to the non-inoculated control. In the second experiment, the ERM of Gi. margarita started to grow after a period of 50 days and reached average densities per treatment of only up to 27 cm cm(-2) by the end of the cultivation. The growth of Gi. margarita mycelium was not inhibited by Cd. No differences in root growth were observed between the Gi. margarita inoculated and non-inoculated treatments. The inhibitory effect of Cd on root growth differed between the non-inoculated treatments in both experiments. The study has shown that the AM fungus Glomus intraradices can alleviate Cd-induced growth inhibition to carrot hairy roots. The potential and limits of the monoxenic system in studying the interaction between AM fungi and heavy metals are discussed.
Zobrazit více v PubMed
Mycorrhiza. 2002 Aug;12(4):181-4 PubMed
Mycorrhiza. 2003 Jun;13(3):151-7 PubMed
J Exp Bot. 2002 May;53(371):1177-85 PubMed
Environ Pollut. 1994;86(2):171-9 PubMed
Plant Physiol. 2002 Nov;130(3):1162-71 PubMed
Environ Pollut. 1997;98(1):29-36 PubMed
FEMS Microbiol Lett. 2002 Jan 2;206(1):39-43 PubMed
