Genetic determinants of folate status in Central Bohemia
Language English Country Czech Republic Media print
Document type Clinical Trial, Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15588157
PII: 602
Knihovny.cz E-resources
- MeSH
- Aryl Hydrocarbon Hydroxylases genetics MeSH
- Genetic Predisposition to Disease epidemiology MeSH
- Genetic Testing methods MeSH
- Risk Assessment methods MeSH
- Folic Acid administration & dosage blood MeSH
- Middle Aged MeSH
- Humans MeSH
- Methylenetetrahydrofolate Dehydrogenase (NAD+) blood genetics MeSH
- DNA Mutational Analysis methods MeSH
- Lung Neoplasms enzymology epidemiology genetics MeSH
- Polymorphism, Genetic MeSH
- Dietary Supplements MeSH
- Risk Factors MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Controlled Clinical Trial MeSH
- Clinical Trial MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Aryl Hydrocarbon Hydroxylases MeSH
- CYP2A13 protein, human MeSH Browser
- Folic Acid MeSH
- Methylenetetrahydrofolate Dehydrogenase (NAD+) MeSH
Although several genetic factors have been implicated as determinants of blood folate concentration in various populations, their effect on folate status in the Czech population has not yet been examined. We explored whether blood folate concentrations in healthy Czech population are associated with polymorphisms in 5,10-methylenetetrahydrofolate reductase (MTHFR), folate hydrolase 1 (FOLH1), reduced folate carrier (RFC), and folate receptor (FOLR1) genes. In a cross-sectional study of 591 control subjects we determined genotypes by PCR-RFLP or ARMS-PCR methods, and plasma and erythrocyte folates by MEIA. The effect of different genotypes on folate status was examined by non-parametric tests and by regression analysis. The prevalence of the MTHFR 677C>T, MTHFR 1298A>C, FOLH1 1561C>T, RFC 80G>A and FOLR1 480G>C variant alleles was 0.34, 0.33, 0.05, 0.44 and 0.00, respectively. Only the MTHFR 677C>T variant was significantly associated with plasma folate concentrations (median 14.7, 14.0 and 12.2 nmol/l for the CC, CT and TT genotypes, respectively). Our study showed that among the five studied allelic variants, only the 677C>T polymorphism in the MTHFR gene is a significant genetic determinant of plasma folate concentrations in Czech population.
