Hypoxia-induced long-term increase of dopamine and tyrosine hydroxylase mRNA levels
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15782555
Knihovny.cz E-resources
- MeSH
- DNA-Binding Proteins metabolism MeSH
- Dopamine genetics metabolism MeSH
- Gene Expression MeSH
- Hypoxia-Inducible Factor 1, alpha Subunit MeSH
- Hypoxia-Inducible Factor 1 MeSH
- Hypoxia metabolism MeSH
- Nuclear Proteins metabolism MeSH
- Rats MeSH
- Cells, Cultured MeSH
- RNA, Messenger metabolism MeSH
- Mesencephalon metabolism MeSH
- Rats, Wistar MeSH
- Transcription Factors metabolism MeSH
- Tyrosine 3-Monooxygenase genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA-Binding Proteins MeSH
- Dopamine MeSH
- Hypoxia-Inducible Factor 1, alpha Subunit MeSH
- Hypoxia-Inducible Factor 1 MeSH
- Hif1a protein, rat MeSH Browser
- Nuclear Proteins MeSH
- RNA, Messenger MeSH
- Transcription Factors MeSH
- Tyrosine 3-Monooxygenase MeSH
The aim of the present study was to determine hypoxia-induced changes in the long-term expression of tyrosine hydroxylase (TH) mRNA and the steady-state dopamine (DA) levels in rat mesencephalic cell cultures. The cultures were exposed to hypoxia during the early developmental period, and DA content and TH mRNA expression were determined on day in vitro (DIV) 14. Hypoxic exposure of 5-day-old cultures resulted in increased DA (control 89.9+/-8.9, hypoxia 135.8+/-23.7 pg/microg protein) and TH mRNA (control 37.3+/-4.7, hypoxia 143.1+/-49.4 pg/microg RNA) levels. To analyze the involvement of hypoxia-inducible factor-1 (HIF-1) in these changes, we studied its activation using reporter gene. Hypoxia caused a 3-fold increase in HIF-1 activity. Our data suggest that hypoxia/ischemia during the putative critical developmental period of neurons may determine the tyrosine hydroxylase gene expression and, consequently, the development of the dopaminergic system.
