Light-dependent PER-like proteins in the cephalic ganglia of an apterygote and a pterygote insect species
Language English Country Germany Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Nucleus metabolism MeSH
- Period Circadian Proteins MeSH
- Circadian Rhythm MeSH
- Cytoplasm metabolism MeSH
- Species Specificity MeSH
- Photoperiod MeSH
- Ganglia cytology metabolism radiation effects MeSH
- Insecta metabolism radiation effects MeSH
- Immunohistochemistry MeSH
- Nuclear Proteins metabolism MeSH
- Brain cytology metabolism MeSH
- Periplaneta metabolism radiation effects MeSH
- Drosophila Proteins MeSH
- Light MeSH
- Protein Transport MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Period Circadian Proteins MeSH
- Nuclear Proteins MeSH
- PER protein, Drosophila MeSH Browser
- Drosophila Proteins MeSH
Antibodies targeted to a highly conserved tetradecapeptide region of the pivotal biological clock protein PER detect in the firebrat Thermobia domestica a 115-kDa protein and in the cockroach Periplaneta americana a 110-kDa protein that are present in the cytoplasm of a small set of brain cells. A similar cytoplasmic reaction occurs with antisera to the whole PER protein of Drosophila melanogaster, but these antisera also react with numerous cell nuclei. On western blots, they detect an 80-kDa antigen in T. domestica and 70- and 80-kDa antigens in P. americana. No indication of antigen translocation between cell nuclei and cytoplasm was found. Nuclear staining is maintained at a high constant level in T. domestica held at a 12:12 h light:dark photoperiod (LD) or in continuous light, but disappears rapidly in response to extended darkness. In P. americana under LD conditions, the number of immunoreactive nuclei and their staining intensity fluctuate in parallel, with maximal staining late in the day. The circadian changes are maintained in continuous light but all staining vanishes in continuous darkness. A 6-h light pulse in early night of an LD cycle induces maximal staining after about 10 h, suggesting that the effect of light on nuclear PER-like expression is indirect. The behaviour of nuclear antigens is opposite to that of the cytoplasmic PER-like proteins that persist in constant darkness and disappear in constant light. Under LD conditions, the cytoplasmic PER-like antigen cycles in T. domestica but remains at a steady level in P. americana. The sensitivity to photoregime suggests that both the nuclear and the cytoplasmic PER-like antigens are components of the biological clock.
Pedagogical Faculty University of South Bohemia Jeronýmova 10 371 15 Ceské Budejovice Czech Republic
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