Comparison of protective effect of protein and DNA vaccines hsp90 in murine model of systemic candidiasis
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
15954537
DOI
10.1007/bf02931297
Knihovny.cz E-zdroje
- MeSH
- analýza přežití MeSH
- Candida albicans imunologie MeSH
- DNA vakcíny aplikace a dávkování imunologie MeSH
- Freundovo adjuvans MeSH
- fungální vakcíny aplikace a dávkování imunologie MeSH
- imunoglobulin G krev MeSH
- injekce intradermální MeSH
- injekce intramuskulární MeSH
- kandidóza prevence a kontrola MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- proteiny tepelného šoku HSP90 aplikace a dávkování genetika imunologie MeSH
- protilátky fungální krev MeSH
- subjednotkové vakcíny aplikace a dávkování imunologie MeSH
- syntetické vakcíny aplikace a dávkování imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- DNA vakcíny MeSH
- Freundovo adjuvans MeSH
- fungální vakcíny MeSH
- imunoglobulin G MeSH
- proteiny tepelného šoku HSP90 MeSH
- protilátky fungální MeSH
- subjednotkové vakcíny MeSH
- syntetické vakcíny MeSH
Preventive vaccination by a hsp90-expressing DNA vaccine and recombinant hsp90 protein vaccine, both derived from the Candida albicans hsp90 using BALB-c mouse model of systemic candidiasis, was performed. Hsp90 mRNA was cloned from a clinical isolate of C. albicans, converted to cDNA and cloned into vaccination plasmid pVAX1. Two methods of DNA application were tested: intramuscular (i.m.) and intradermal (i.d.) injection. Recombinant protein was applied by i.d. injection with Freund's adjuvant; the control groups received PBS or Freund's adjuvant only. Mice were vaccinated and after 19 d re-vaccinated. After 3 weeks, the mice were challenged with the live C. albicans in a dose of 5 x 10(6) CFU per mouse. After the challenge, the mice vaccinated i.d. with DNA vaccine survived for 39 and 64% longer compared to those receiving Freund's adjuvant and/or PBS, respectively. The i.m. application of the DNA vaccine did not provide any significant protectivity. The serum level of anti-candida-hsp90 serum IgG antibodies correlated with the survival rate in both i.d. protein and DNA vaccination approaches. We stressed the importance of specific humoral immunity in the mouse model of systemic candidiasis.
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Candidiasis--do we need to fight or to tolerate the Candida fungus?
