Development of high-performance liquid chromatographic determination of salicylaldehyde isonicotinoyl hydrazone in rabbit plasma and application of this method to an in vivo study
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Validation Study
- MeSH
- Aldehydes blood MeSH
- Blood Chemical Analysis methods standards statistics & numerical data MeSH
- Iron Chelating Agents analysis MeSH
- Hydrazones blood MeSH
- Rabbits MeSH
- Quality Control MeSH
- Drug Stability MeSH
- Chromatography, High Pressure Liquid methods standards statistics & numerical data MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Validation Study MeSH
- Names of Substances
- Aldehydes MeSH
- Iron Chelating Agents MeSH
- Hydrazones MeSH
- salicylaldehyde isonicotinoyl hydrazone MeSH Browser
An analytical methodology appropriate for the determination of the novel drug candidate salicylaldehyde isonicotinoyl hydrazone (SIH) in rabbit plasma has been developed and validated. Desirable chromatographic separation was achieved on a C18 column employing a mixture of phosphate buffer (0.01 M NaH2PO4 x 2 H2O with 2 mM EDTA, pH 6.0) and methanol (53:47; v/v) as the mobile phase. In order to develop a suitable sample preparation procedure, different methods have been tested (solid-phase extraction, liquid-liquid extraction, and protein precipitation). Protein precipitation using 0.1 M HClO4 and acetonitrile allowed the highest recoveries of the analyte to be reproducibly attained. The analytical methodology developed in this study was validated with respect to linearity (0.26-30.0 microg/mL), accuracy, precision, selectivity, recovery, and stability. A concentration of 0.26 microg/mL was determined as the LLOQ. The chromatographic method was applied to a preliminary plasma pharmacokinetic study. This study has provided the first information about the concentrations of SIH in plasma of a living subject. These results could have a significant impact on further progress in the development of this promising compound.
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