A method of meiotic segregation analysis based on recombinant selection in the homothallic basidiomycete Phanerochaete chrysosporium was developed. Using this method, we were able to reveal linkage relationships and to estimate recombination frequencies between seven mutations to auxotrophy. We detected two linkage groups, the first containing four and the second three of the seven mapped mutations.

Institute of Biotechnology Charles University Vinicná 5 128 44 Prague 2 and Institute of Microbiology Czechoslovak Academy of Sciences Vídenská 1083 142 20 Prague 4 Czechoslovakia

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