A novel iron responsive element in the 3'UTR of human MRCKalpha
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16412980
DOI
10.1016/j.bbrc.2005.12.155
PII: S0006-291X(05)02932-3
Knihovny.cz E-resources
- MeSH
- 3' Untranslated Regions genetics MeSH
- K562 Cells MeSH
- Antigens, CD metabolism MeSH
- Myotonin-Protein Kinase MeSH
- Humans MeSH
- Protein Serine-Threonine Kinases genetics metabolism MeSH
- Iron-Regulatory Proteins genetics metabolism MeSH
- Receptors, Transferrin metabolism MeSH
- Iron pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 3' Untranslated Regions MeSH
- Antigens, CD MeSH
- CD71 antigen MeSH Browser
- CDC42BPA protein, human MeSH Browser
- Myotonin-Protein Kinase MeSH
- Protein Serine-Threonine Kinases MeSH
- Iron-Regulatory Proteins MeSH
- Receptors, Transferrin MeSH
- Iron MeSH
Human untranslated region (UTR) databases were searched to identify novel proteins potentially regulated by an iron responsive element (IRE), and found two candidates-cell cycle phosphatase Cdc14A variant 1 and myotonic dystrophy kinase-related Cdc42-binding kinase alpha (MRCKalpha), both possessing a putative IRE in their 3'UTR. In further experiments, we focused on MRCKalpha. Biochemical analyses of the MRCKalpha IRE revealed that it was functional and mediated the response to iron level in the same way as transferrin receptor 1 IREs (TfR) did. Similarly to TfR mRNA, MRCKalpha mRNA is stabilized, when iron supply is low, while it is destabilized under iron-rich conditions. The expression of MRCKalpha mRNA was found to be ubiquitous; the highest levels were noted in testes, the lowest in skeletal muscle. The level of MRCKalpha mRNA in various tissues strongly positively correlates with the level of TfR mRNA, indicating its possible role in the transferrin iron uptake pathway.
References provided by Crossref.org
