Immunohistochemical analysis of the biological potential of odontogenic keratocysts
Jazyk angličtina Země Dánsko Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
16430736
DOI
10.1111/j.1600-0714.2006.00382.x
PII: JOP382
Knihovny.cz E-zdroje
- MeSH
- antigen Ki-67 analýza MeSH
- apoptóza fyziologie MeSH
- biologické markery analýza MeSH
- biologie MeSH
- dentigerózní cysta chemie patologie MeSH
- diferenciální diagnóza MeSH
- epitel chemie patologie MeSH
- imunofenotypizace MeSH
- imunohistochemie MeSH
- inhibitor p27 cyklin-dependentní kinasy analýza MeSH
- inhibitory proteinkinas analýza MeSH
- lidé MeSH
- odontogenní cysty chemie patologie MeSH
- prognóza MeSH
- proliferace buněk MeSH
- proteiny buněčného cyklu analýza MeSH
- proteiny regulující apoptózu analýza MeSH
- protoonkogenní proteiny c-bcl-2 analýza MeSH
- radikulární cysta chemie patologie MeSH
- receptor erbB-2 analýza MeSH
- syndrom bazocelulárního névu metabolismus patologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- antigen Ki-67 MeSH
- biologické markery MeSH
- inhibitor p27 cyklin-dependentní kinasy MeSH
- inhibitory proteinkinas MeSH
- proteiny buněčného cyklu MeSH
- proteiny regulující apoptózu MeSH
- protoonkogenní proteiny c-bcl-2 MeSH
- receptor erbB-2 MeSH
BACKGROUND: The aim of this study was to analyse the usefulness of detecting important apoptosis and proliferation markers in assessing the biological potential of odontogenic keratocysts (OKC) and thus selecting the optimal diagnostic algorithm for these lesions. METHODS: Indirect immunohistochemistry and relevant statistical methods were used for analysis of formalin-fixed and paraffin-embedded samples from 98 patients. RESULTS: Nevoid basal cell carcinoma syndrome (NBCCS) keratocysts were characterized by higher expression of Bcl-2, p27Kip1 and c-erbB-2 as well as by lower proliferative activity measured by Ki-67 in basal cell epithelium and by a lower inflammatory response in comparison with sporadic keratocysts. Dentigerous, radicular and non-specified odontogenic cysts differed from both NBCCS and sporadic keratocysts in a wide spectrum of apoptosis and/or cell cycle-related protein expressions, higher proliferation in the basal cell layer, and vice versa, lower proliferation in the suprabasal cell layer. CONCLUSIONS: The NBCCS keratocysts have a different immunophenotype from sporadic keratocysts and both types are distinguishable from dentigerous, radicular and non-specified odontogenic cysts. These findings confirm the separate biological potential of these lesions and the results of the immunohistochemical analysis have diagnostic and prognostic implications.
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