The effect of down-regulation of mitochondrial RNA-binding proteins MRP1 and MRP2 on respiratory complexes in procyclic Trypanosoma brucei
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
5R03TW6445-2
FIC NIH HHS - United States
PubMed
16730807
DOI
10.1016/j.molbiopara.2006.04.007
PII: S0166-6851(06)00139-3
Knihovny.cz E-resources
- MeSH
- Down-Regulation MeSH
- RNA Editing MeSH
- Electron Transport Chain Complex Proteins metabolism MeSH
- Mitochondrial Proteins genetics metabolism MeSH
- Mitochondria metabolism MeSH
- RNA-Binding Proteins genetics metabolism MeSH
- Proton-Translocating ATPases genetics MeSH
- Electron Transport Complex IV genetics metabolism MeSH
- RNA Interference MeSH
- Electron Transport MeSH
- Trypanosoma brucei brucei genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Names of Substances
- Electron Transport Chain Complex Proteins MeSH
- Mitochondrial Proteins MeSH
- RNA-Binding Proteins MeSH
- Proton-Translocating ATPases MeSH
- Electron Transport Complex IV MeSH
MRP1 and MRP2 are multifunctional mitochondrial RNA-binding proteins with a regulatory role in RNA editing and putative role(s) in RNA processing in Trypanosoma brucei. Silencing of MRP1 and/or MRP2 by RNA interference affected the assembly and functionality of respiratory complexes. The absence of several subunits of complexes I, III and IV resulted in their disintegration and subsequent decrease of specific activities and also caused a significant decrease of membrane potential. The overall respiration in the interfered cells decreased by only about 20%, since the trypanosome alternative oxidase effectively replaced the missing cytochromes and became the principal terminal oxidase.
References provided by Crossref.org
Integrity of the core mitochondrial RNA-binding complex 1 is vital for trypanosome RNA editing
