Aspects of protein and amino acid metabolism in a model of severe glutamine deficiency in sepsis
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16809904
DOI
10.1159/000094300
PII: 94300
Knihovny.cz E-resources
- MeSH
- Amino Acids metabolism MeSH
- Glutamine analysis deficiency metabolism MeSH
- Muscle, Skeletal metabolism MeSH
- Rats MeSH
- Random Allocation MeSH
- Rats, Wistar MeSH
- Growth Hormone pharmacology MeSH
- Sepsis metabolism physiopathology MeSH
- Muscle Proteins metabolism MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amino Acids MeSH
- Glutamine MeSH
- Growth Hormone MeSH
- Muscle Proteins MeSH
BACKGROUND/AIMS: Growth hormone (GH) could have the potential to improve protein metabolism in sepsis but glutamine deficiency has been reported after GH treatment. The aim was to investigate the effects of glutamine deficiency in sepsis with and without GH treatment on protein and amino acid metabolism. METHODS: Cecal ligation and puncture (CLP) was used as a model of sepsis. Serious glutamine deficiency was induced by administration of glutamine synthetase inhibitor, methionine sulfoximine (MSO). Young Wistar rats were divided into 5 groups: control; CLP; CLP+MSO; CLP+GH, and CLP+MSO+GH. Parameters of protein metabolism were measured on incubated soleus and extensor digitorum longus muscles: [1-14C]leucine was used to estimate protein synthesis and leucine oxidation, tyrosine release was used to evaluate protein breakdown. Amino acid concentrations in plasma, skeletal muscle and incubation media were measured by HPLC. RESULTS/CONCLUSIONS: A reduced muscle glutamine concentration after MSO treatment is not associated with changes in the rates of protein synthesis or breakdown. MSO treatment decreased glutamine release from skeletal muscle and plasma glutamine concentration. Severe glutamine deficiency in GH-treated septic rats resulted in increased release of branched-chain amino acids from skeletal muscle.
References provided by Crossref.org
The dose-dependent effects of endotoxin on protein metabolism in two types of rat skeletal muscle
Muscle wasting in animal models of severe illness
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