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PubMed

Záznam pochází z PubMed

Effects of β-hydroxy-β-methylbutyrate treatment in different types of skeletal muscle of intact and septic rats

Journal of physiology and biochemistry. 2010 Dec ; 66 (4) : 311-9. [epub] 20100820

J Physiol Biochem
ISSN 1138-7548
Zdroj

Jazyk angličtina Země Španělsko Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz https://www.medvik.cz/link/pmid20725872

PubMed 20725872
DOI 10.1007/s13105-010-0037-3
Knihovny.cz E-zdroje

MeSH
časové faktory MeSH
kathepsin B chemie MeSH
kathepsin L chemie MeSH
kosterní svaly účinky léků metabolismus MeSH
krysa rodu Rattus MeSH
kyslík chemie MeSH
leucin chemie MeSH
lyzozomy metabolismus MeSH
potkani Wistar MeSH
proteasomový endopeptidasový komplex chemie MeSH
sepse farmakoterapie metabolismus MeSH
valeráty farmakologie MeSH
zvířata MeSH
Check Tag
krysa rodu Rattus MeSH
mužské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
beta-hydroxyisovaleric acid MeSH Prohlížeč
kathepsin B MeSH
kathepsin L MeSH
kyslík MeSH
leucin MeSH
proteasomový endopeptidasový komplex MeSH
valeráty MeSH

β-Hydroxy-β-methylbutyrate (HMB) is a leucine metabolite that may have a positive effect in protein catabolic conditions. Therefore, we hypothesized that HMB treatment could attenuate the sepsis-induced protein catabolic state. The aims of our study were to elucidate the effect of HMB in healthy and septic animals and to evaluate the differences in the action of HMB in different muscle types. Intact and septic (5 mg endotoxin/kg i.p.) rats were administered with HMB (0.5 g/kg/day) or saline. After 24 h, extensor digitorum longus (EDL) and soleus (SOL) muscles were isolated and used for determination of total and myofibrillar proteolysis, protein synthesis, leucine oxidation, activity of cathepsins B and L, chymotrypsin-like activity, and expression of α-subunits of proteasome. Our results indicate that the catabolic state induced by the endotoxin treatment was caused both by increase in protein breakdown (due to activation of proteasome system) and by attenuation of protein synthesis. The EDL (muscle composed of white, fast-twitch fibers) was more susceptible to these changes than the SOL (muscle composed of red, slow-twitch fibers). The HMB treatment had no effect in healthy animals but counteracted the changes in septic animals. The action of HMB was mediated by attenuation of proteasome activity and protein breakdown, not by stimulation of protein synthesis. More pronounced effect of the HMB treatment on myofibrillar proteolysis was observed in the SOL.

Department of Physiology Faculty of Medicine Charles University Simkova 870 500 38 Hradec Kralove Czech Republic

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