IgE-mediated allergy is a common chronic disorder resulting from interactions between genetic and environmental factors. The gene encoding CD14 is a positional candidate gene for allergic diseases as it is localised on chromosome 5q31.1, a region linked to asthma and bronchial hyperresponsiveness. We investigated the relationship among atopic phenotypes and six polymorphisms in the CD14 gene. Polymerase chain reaction with RFLP analyses was used to determine the CD14 genotypes in subjects with IgE-mediated allergic diseases (n=282) and random controls (n=187). No significant differences in allele or genotype frequencies for individual polymorphisms between patients and controls were found. However, when atopic patients were subdivided into subjects with positive and with negative skin prick tests for separate antigens, T allele of the 1341G/T polymorphism was significantly associated with positive reactivity to mites (P=0.007) and moulds (P=0.041). Similarly, the C allele frequency of the -159C/T variant was increased in patients with positive skin prick tests for mites (P=0.046) and moulds (P=0.056). In haplotype analysis, the common -1619A/-1359G/-550C/-159C/+1188G/+1341T haplotype was associated with positive reaction to these antigens (P values: 0.0008-0.0035). Our study supports the idea that CD14 plays a role in IgE-mediated allergic diseases, and its gene polymorphisms can be important for manifestation of these disorders.

Institute of Pathological Physiology LF MU Medical Faculty Masaryk University Komenskeho nam 2 Brno 662 43 Czech Republic

Zobrazit více v PubMed

Am J Respir Crit Care Med. 2001 Mar;163(4):965-9 PubMed

Hum Mutat. 2000 Jan;15(1):122 PubMed

Int Arch Allergy Immunol. 2001 Jan-Mar;124(1-3):20-4 PubMed

Am J Cardiol. 2000 Sep 15;86(6):682-4, A8 PubMed

Am J Respir Crit Care Med. 2002 Feb 15;165(4):551-2; author reply 552 PubMed

Am J Respir Crit Care Med. 2006 Mar 15;173(6):617-22 PubMed

J Investig Allergol Clin Immunol. 2002;12(3):192-7 PubMed

Am J Physiol. 1996 May;270(5 Pt 1):L736-44 PubMed

J Endotoxin Res. 2001;7(1):45-8 PubMed

Curr Opin Immunol. 1999 Dec;11(6):606-9 PubMed

Semin Respir Crit Care Med. 2002 Aug;23(4):317-29 PubMed

Allergy. 2002 Mar;57(3):188-92 PubMed

J Immunol. 1988 Jul 15;141(2):547-52 PubMed

J Hum Genet. 2006;51(1):59-67 PubMed

Pediatr Allergy Immunol. 2003 Aug;14(4):255-60 PubMed

Am J Respir Cell Mol Biol. 1999 May;20(5):976-83 PubMed

Clin Exp Pharmacol Physiol. 2006 Mar;33(3):285-9 PubMed

Am J Hum Genet. 2000 Nov;67(5):1154-62 PubMed

Clin Genet. 1999 Aug;56(2):164-5 PubMed

Allergy. 2004 May;59(5):520-5 PubMed

Allergy. 2003 Oct;58(10):1023-6 PubMed