An invertebrate TNF functional analogue activates macrophages via lectin-saccharide interaction with ion channels
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17035350
DOI
10.1093/intimm/dxl100
PII: dxl100
Knihovny.cz E-resources
- MeSH
- Macrophage Activation drug effects immunology MeSH
- Disaccharides metabolism MeSH
- Interleukin-6 metabolism MeSH
- Ion Channels metabolism MeSH
- Lectins chemistry metabolism pharmacology MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Sodium-Hydrogen Exchangers genetics metabolism MeSH
- NF-kappa B metabolism MeSH
- Nitric Oxide metabolism MeSH
- Macrophages, Peritoneal immunology metabolism MeSH
- Signal Transduction MeSH
- Tumor Necrosis Factor-alpha metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- coelomic cytolytic factor 1, Eisenia fetida MeSH Browser
- Disaccharides MeSH
- Interleukin-6 MeSH
- Ion Channels MeSH
- Lectins MeSH
- N,N-diacetylchitobiose MeSH Browser
- Sodium-Hydrogen Exchangers MeSH
- NF-kappa B MeSH
- Nitric Oxide MeSH
- Tumor Necrosis Factor-alpha MeSH
The invertebrate pattern-recognition protein named coelomic cytolytic factor (CCF) and the mammalian cytokine tumor necrosis factor (TNF) share functional analogies that are based on a similar saccharide recognition specificity. In particular, CCF and TNF have been shown to interact with ion channels on the surface of vertebrate cells via N,N'-diacetylchitobiose lectin-like activity. In the present study, we show that CCF-induced membrane depolarization results in the release of TNF, IL-6 and nitric oxide (NO) by macrophages via nuclear factor-kappaB signaling. Interestingly, our data suggest that TNF contributes, through lectin-saccharide interaction, to the secretion of IL-6 and NO induced by CCF. This experimental non-physiological setting based on the interaction of an invertebrate defense lectin with vertebrate cells involved in the innate immune response may have highlighted an evolutionarily ancient mechanism of macrophage activation in vertebrates.
References provided by Crossref.org
Soluble mediators of innate immunity in annelids and bivalve mollusks: A mini-review
