The fission yeast ortholog of eIF3a subunit is not functional in Saccharomyces cerevisiae
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17455792
DOI
10.1007/bf02931620
Knihovny.cz E-resources
- MeSH
- Cytoplasm chemistry MeSH
- Gene Deletion MeSH
- Eukaryotic Initiation Factor-3 genetics physiology MeSH
- Microscopy, Fluorescence MeSH
- Cloning, Molecular MeSH
- Microscopy, Confocal MeSH
- Protein Biosynthesis genetics MeSH
- Ribosomes metabolism MeSH
- Saccharomyces cerevisiae genetics growth & development physiology MeSH
- Schizosaccharomyces pombe Proteins genetics physiology MeSH
- Schizosaccharomyces genetics MeSH
- Genetic Complementation Test MeSH
- Protein Binding MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Eukaryotic Initiation Factor-3 MeSH
- Schizosaccharomyces pombe Proteins MeSH
The Schizosaccharomyces pombe eIF3a ortholog (SpeIF3a) was shown to be unable to substitute for S. cerevisiae eIF3a (SceIF3a) in its essential function in the initiation of translation. Overproduction of SpeIF3a altered the distribution of SceIF3a but formation of the endogenous eIF3 complex was not affected. SpeIF3a was found to be more tightly bound to S. cerevisiae ribosomes than SceIF3a and other eIF3 subunits (eIF3g, eIF3i, eIF3j). The host cells displayed aberrant morphology and altered chitin deposition. SpeIF3a probably competes with SceIF3a for binding to either ribosomes or yet to be identified substrates.
See more in PubMed
Mol Biol Cell. 1996 Dec;7(12):1909-19 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
J Biol Chem. 1994 Apr 1;269(13):9833-41 PubMed
Folia Biol (Praha). 1998;44(2):73 PubMed
Mol Gen Genet. 1999 Jun;261(4-5):820-30 PubMed
J Biol Chem. 2001 Jan 19;276(3):2122-31 PubMed
Genetics. 1999 Sep;153(1):35-47 PubMed
Methods. 1997 Apr;11(4):343-52 PubMed
Genes Dev. 2003 Mar 15;17(6):786-99 PubMed
Curr Genet. 1998 Feb;33(2):100-9 PubMed
Cell Motil Cytoskeleton. 2000 Mar;45(3):235-46 PubMed
Eur J Biochem. 2003 Oct;270(20):4133-9 PubMed
Nucleic Acids Res. 1988 Jul 11;16(13):6127-45 PubMed
Biotechnology (N Y). 1990 Mar;8(3):223-7 PubMed
EMBO J. 1999 Mar 15;18(6):1673-88 PubMed
J Biol Chem. 1998 Aug 14;273(33):21253-60 PubMed
Trends Biochem Sci. 1998 Jun;23(6):204-5 PubMed
J Biol Chem. 1999 Sep 24;274(39):27567-72 PubMed
Genetics. 2001 Apr;157(4):1425-36 PubMed
Mol Cell Biol. 1998 Aug;18(8):4935-46 PubMed
EMBO J. 2002 Nov 1;21(21):5886-98 PubMed
J Cell Biol. 1993 Aug;122(3):635-44 PubMed
Methods Mol Biol. 2006;313:85-96 PubMed
J Biol Chem. 2001 Mar 30;276(13):10056-62 PubMed
Biochem Biophys Res Commun. 2001 Apr 20;282(5):1244-50 PubMed
Gene. 1985;33(1):103-19 PubMed
EMBO J. 1983;2(12):2161-8 PubMed
J Biol Chem. 1994 Dec 23;269(51):32286-92 PubMed
J Cell Biol. 1993 Jan;120(1):55-65 PubMed
Yeast. 1999 Jul;15(10A):865-72 PubMed
J Biol Chem. 1998 Jul 17;273(29):18573-85 PubMed
Gene. 1987;52(2-3):225-33 PubMed
J Biol Chem. 1997 Jan 10;272(2):1110-6 PubMed
