Antibody-quantum dot conjugates exhibit enhanced antibacterial effect vs. unconjugated quantum dots
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
17571792
DOI
10.1007/bf02932134
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Biotechnology methods MeSH
- Escherichia coli drug effects growth & development MeSH
- Immunoconjugates MeSH
- Quantum Dots * MeSH
- Microbial Sensitivity Tests methods MeSH
- Antibodies, Monoclonal * chemistry immunology pharmacology MeSH
- Colony Count, Microbial MeSH
- Antibodies, Bacterial * chemistry immunology pharmacology MeSH
- Salmonella typhimurium drug effects growth & development MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Immunoconjugates MeSH
- Antibodies, Monoclonal * MeSH
- Antibodies, Bacterial * MeSH
The effect of a 20-min exposure to antibody-quantum dot (Ab-QD) conjugates on colony counts of Escherichia coli was assessed by the spread-plate method and compared with exposure to unconjugated QDs having only amine or carboxyl groups on their surfaces. Under these conditions, Ab-QD conjugates generally exhibited >90% reduction in colony-forming units as compared to untreated E. coli and E. coli treated with unconjugated QDs after incubation for as long as 41 h. The antibacterial effect of Ab-QD conjugates vs. unconjugated QDs on Salmonella enterica subsp. enterica serovar Typhimurium was also assessed by means of a disk-diffusion technique which demonstrated greater growth inhibition (approximately 3 mm greater) by Ab-QD conjugate-impregnated disks than by unconjugated-QD-only-impregnated disks at a 10-microg disk load. At a 25-microg disk load, both treatment groups exhibited nearly equal growth inhibition.
See more in PubMed
Appl Environ Microbiol. 2003 Jul;69(7):4205-13 PubMed
Toxicology. 2005 Sep 15;213(1-2):66-73 PubMed
Environ Health Perspect. 2006 Feb;114(2):165-72 PubMed
Nat Biotechnol. 2004 Nov;22(11):1360-1 PubMed
Biochem Biophys Res Commun. 2004 Dec 17;325(3):739-43 PubMed
Appl Environ Microbiol. 2005 May;71(5):2548-57 PubMed
J Am Chem Soc. 2006 May 17;128(19):6278-9 PubMed
Microbiol Immunol. 2004;48(9):669-75 PubMed
In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexes
