In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexes
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, Research Support, U.S. Gov't, Non-P.H.S.
- MeSH
- antibakteriální látky chemie imunologie farmakologie MeSH
- aptamerová technika SELEX MeSH
- aptamery nukleotidové chemie genetika imunologie farmakologie MeSH
- Escherichia coli účinky léků imunologie MeSH
- infekce vyvolané Escherichia coli farmakoterapie imunologie MeSH
- komplement C1 chemie imunologie MeSH
- lidé MeSH
- lipopolysacharidy chemie imunologie MeSH
- molekulární sekvence - údaje MeSH
- sekvence nukleotidů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Názvy látek
- antibakteriální látky MeSH
- aptamery nukleotidové MeSH
- komplement C1 MeSH
- lipopolysacharidy MeSH
DNA aptamers were developed against lipopolysaccharide (LPS) from E. coli O111:B4 and shown to bind both LPS and E. coli by a colorimetric enzyme-based microplate assay. The polyclonal aptamers were coupled to human C1qrs protein either directly using a bifunctional linker or indirectly using biotinylated aptamers and a streptavidin-C1qrs complex. Both systems significantly reduced colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10x dilutions of the bacteria in the presence of human serum; it was diluted 1: 10(3) in order to avoid significant bacterial lysis by the competing alternate pathway of complement activation. A number of candidate DNA aptamer sequences were cloned and sequenced from the anti-LPS aptamer library for future screening of antibacterial or "antibiotic" potential and to aid in eventual development of an alternative therapy for antibiotic-resistant bacterial infections.
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