Ultrafiltered pig leukocyte extract (IMUNOR) decreases nitric oxide formation and hematopoiesis-stimulating cytokine production in lipopolysaccharide-stimulated RAW 264.7 macrophages
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17673152
DOI
10.1016/j.intimp.2007.06.003
PII: S1567-5769(07)00166-X
Knihovny.cz E-resources
- MeSH
- Cell Extracts MeSH
- Cell Line MeSH
- Bone Marrow Cells cytology drug effects MeSH
- Cytokines metabolism MeSH
- Granulocyte Colony-Stimulating Factor metabolism pharmacology MeSH
- Hematopoiesis drug effects MeSH
- Immunologic Factors pharmacology MeSH
- Mice, Inbred Strains MeSH
- Cells, Cultured MeSH
- Lipopolysaccharides MeSH
- Macrophages drug effects metabolism MeSH
- Mice MeSH
- Nitric Oxide metabolism MeSH
- Swine MeSH
- Tissue Extracts pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cell Extracts MeSH
- Cytokines MeSH
- Granulocyte Colony-Stimulating Factor MeSH
- Immunologic Factors MeSH
- IMUNOR MeSH Browser
- Lipopolysaccharides MeSH
- Nitric Oxide MeSH
- Tissue Extracts MeSH
A low-molecular-weight (<12 kDa) ultrafiltered pig leukocyte extract, IMUNOR, was tested in experiments in vitro on non-stimulated and lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages in order to assess modulation of nitric oxide (NO) production (measured indirectly as the concentration of nitrites), hematopoiesis-stimulating activity of the supernatant of the macrophage cells (ascertained by counting cell colonies growing from progenitor cells for granulocytes and macrophages (GM-CFC) in vitro), and the release of hematopoiesis-stimulating cytokines. No hematopoiesis-stimulating activity and cytokine or NO production were found in the supernatant of non-stimulated macrophages. It was found that IMUNOR does not influence this status. Supernatant of LPS-stimulated macrophages was characterized by hematopoiesis-stimulating activity, as well as by the presence of nitrites, interleukin-6 (IL-6), and granulocyte colony-stimulating factor (G-CSF). A key role in the hematopoiesis-stimulating activity of the supernatant of LPS-stimulated macrophages could be ascribed to G-CSF since the formation of the colonies could be abrogated nearly completely by monoclonal antibodies against G-CSF. IMUNOR was found to suppress all the mentioned manifestations of the LPS-activated macrophages. When considering these results together with those from our previous in vivo study revealing stimulatory effects of IMUNOR on radiation-suppressed hematopoiesis, a hypothesis may be formulated which postulates a homeostatic role of IMUNOR, consisting in stimulation of impaired immune and hematopoietic systems but also in cutting back the production of proinflammatory mediators in cases of overstimulation which threats with undesirable consequences.
References provided by Crossref.org
Physicochemical Characterization of the Oral Biotherapeutic Drug IMUNOR®
The role of adenosine receptor agonists in regulation of hematopoiesis
