Crystallization and preliminary X-ray diffraction analysis of the small laccase from Streptomyces coelicolor
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18084099
PubMed Central
PMC2344102
DOI
10.1107/s1744309107060721
PII: S1744309107060721
Knihovny.cz E-resources
- MeSH
- X-Ray Diffraction MeSH
- Crystallization MeSH
- Laccase chemistry genetics metabolism MeSH
- Molecular Weight MeSH
- Streptomyces coelicolor enzymology genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Laccase MeSH
The small bacterial laccase from the actinobacterium Streptomyces coelicolor which lacks the second of the three domains of the laccases structurally characterized to date was crystallized. This multi-copper phenol oxidase crystallizes in a primitive tetragonal lattice, with unit-cell parameters a = b = 179.8, c = 175.3 A. The crystals belong to either space group P4(1)2(1)2 or P4(3)2(1)2. The self-rotation function shows the presence of a noncrystallographic threefold axis in the structure. Phases will be determined from the anomalous signal of the natively present copper ions.
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Christensen, T., Woeldike, H., Boel, E., Mortensen, S. B., Hjortshoej, K., Thim, L. & Hansen, M. T. (1988). Nature Biotechnol.6, 1419–1422.
Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760–763. PubMed
Couto, S. R. & Herrera, J. L. T. (2006). Biotechnol. Adv.24, 500–513. PubMed
Cudney, R., Patel, S., Weisgraber, K., Newhouse, Y. & McPherson, A. (1994). Acta Cryst. D50, 414–423. PubMed
Ducros, V., Brzozowski, A. M., Wilson, K. S., Østergaard, P., Schneider, P., Svendson, A. & Davies, G. J. (2001). Acta Cryst. D57, 333–336. PubMed
Jancarik, J. & Kim, S.-H. (1991). J. Appl. Cryst.24, 409–411.
Leslie, A. G. W. (1992). Jnt CCP4/ESF–EACBM Newsl. Protein Crystallogr.26
Machczynski, M. C., Vijgenboom, E., Samyn, B. & Canters, G. W. (2004). Protein Sci.13, 2388–2397. PubMed PMC
Malmström, B. G. (1982). Annu. Rev. Biochem.51, 21–59. PubMed
Mayer, A. M. & Staples, R. C. (2002). Phytochemistry, 60, 551–565. PubMed
Riva, S. (2006). Trends Biotechnol.24, 219–226. PubMed
Sakurai, T. & Kataoka, K. (2007). Chem. Rec.7, 220–229. PubMed
Schneider, T. R. & Sheldrick, G. M. (2002). Acta Cryst. D58, 1772–1779. PubMed
Sheldrick, G. M. (2002). Z. Kristallogr.217, 644–650.
Sheldrick, G. M. (2004). SHELX. http://shelx.uni-ac.gwdg.de/SHELX/.
Vagin, A. & Teplyakov, A. (1997). J. Appl. Cryst.30, 1022–1025.
Weiss, M. S. (2001). J. Appl. Cryst.34, 130–135.
Wells, A., Teria, M. & Eve, T. (2006). Biochem. Soc. Trans.34, 304–308. PubMed
