Hydrophilic interaction liquid chromatography method for the determination of ascorbic acid
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Chromatography, Liquid methods standards MeSH
- Indicators and Reagents MeSH
- Hydrogen-Ion Concentration MeSH
- Ascorbic Acid analysis metabolism standards MeSH
- Chlorogenic Acid analysis standards MeSH
- Oxygen MeSH
- Humans MeSH
- Reference Standards MeSH
- Solvents MeSH
- Spectrophotometry, Ultraviolet MeSH
- Drug Stability MeSH
- Temperature MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Phase Transition MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Indicators and Reagents MeSH
- Ascorbic Acid MeSH
- Chlorogenic Acid MeSH
- Oxygen MeSH
- Solvents MeSH
Hydrophilic interaction liquid chromatography (HILIC) method using internal standard for the determination and stability study of ascorbic acid was developed. HILIC method was very fast and simple using the following analytical conditions: ZIC HILIC (150 x 2.1 mm, 3.5 microm) chromatographic column and mobile phase composed of ACN and 50 mM ammonium acetate buffer pH 6.8 (78:22 v/v). Diode array detection was performed and chromatograms were processed at 268 nm, the maximum wavelength of absorbance of ascorbic acid. An extensive stability study of ascorbic acid as a function of various factors including temperature, stabilizing agents, oxygen presence and its concentration in solution was performed in order to gain information about the quantitative influence of individual stability factors. Low temperature and stabilizing agents (o-phosphoric acid and oxalic acid) were found to be key factors enabling substantial enhancement of the stability of ascorbic acid.
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