Determination of some phenolic acids in Majorana hortensis by capillary electrophoresis with online electrokinetic preconcentration
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18473473
DOI
10.1021/jf8000179
Knihovny.cz E-resources
- MeSH
- Borates MeSH
- Origanum chemistry MeSH
- Electrophoresis, Capillary methods MeSH
- Hydroxybenzoates analysis MeSH
- Hydrogen-Ion Concentration MeSH
- Coumaric Acids analysis MeSH
- Plant Leaves chemistry MeSH
- Plant Extracts chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Borates MeSH
- Hydroxybenzoates MeSH
- Coumaric Acids MeSH
- Plant Extracts MeSH
- sodium borate MeSH Browser
An online accumulation/mobilization preconcentration technique based on a dynamic pH junction technique and electrokinetic injection was employed for analysis of phenolic acids (sinapic, ferulic, coumarinic, caffeic, syringic, vanillic, and 4-hydroxybenzoic acid) in extracts from Majorana hortensis leaves. Samples were extracted by pressurized solvent extraction with acetone at 150 degrees C and 15 MPa. The capillary electrophoretic method employed 50 mmol.L (-1) sodium borate, pH 9.5, as the sample electrolyte, 50 mmol.L (-1) sodium phosphate, pH 2.5, as the background electrolyte, and 50 mmol.L (-1) sodium phosphate, pH 2.5, with 60 mmol.L (-1) sodium dodecyl sulfate as the mobilization electrolyte. The method allowed 720-fold to 5560-fold preconcentration of the phenolic acids during 30 min of electrokinetic accumulation with detection limits from 0.38 to 4.22 ng.mL (-1).
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