Genetic diversity among and within cultured cyanobionts of diverse species of Azolla
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bacterial Proteins genetics metabolism MeSH
- Phylogeny MeSH
- Genetic Variation * MeSH
- Ferns microbiology MeSH
- Culture Techniques MeSH
- Molecular Sequence Data MeSH
- Cyanobacteria classification genetics isolation & purification metabolism MeSH
- Symbiosis * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Bacterial Proteins MeSH
The cyanobionts isolated from 10 Azolla accessions belonging to 6 species (Azolla mexicana, A. microphylla, A. rubra, A. caroliniana, A. filiculoides, A. pinnata) were cultured under laboratory conditions and analyzed on the basis of whole cell protein profiles and molecular marker dataset generated using repeat sequence primers (STRR(mod) and HipTG). The biochemical and molecular marker profiles of the cyanobionts were compared with those of the free-living cyanobacteria and symbiotic Nostoc strains from Anthoceros sp., Cycas sp. and Gunnera monoika. Cluster analysis revealed the genetic diversity among the selected strains, and identified 3 distinct clusters. Group 1 included cyanobionts from all the 10 accessions of Azolla, group 2 comprised all the symbiotic Nostoc strains, while group 3 included the free-living cyanobacteria belonging to the genera Nostoc and Anabaena. The interrelationships among the Azolla cyanobionts were further revealed by principal component analysis. Cyanobionts from A. caroliniana-A. microphylla grouped together while cyanobionts associated with A. mexicana-A. filiculoides along with A. pinnata formed another group. A. rubra cyanobionts had intermediate relationship with both the subgroups. This is the first study analyzing the diversity existing among the cultured cyanobionts of diverse Azolla species through the use of biochemical and molecular profiles and also the genetic distinctness of these free-living cyanobionts as compared to cyanobacterial strains of the genera Anabaena and Nostoc.
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Plant Physiol. 1974 Jun;53(6):813-9 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
Folia Microbiol (Praha). 2006;51(1):50-6 PubMed
FEMS Microbiol Lett. 2003 Dec 5;229(1):43-7 PubMed
Folia Microbiol (Praha). 2006;51(5):455-8 PubMed
Mol Microbiol. 1993 Jan;7(2):189-95 PubMed
Appl Environ Microbiol. 1990 May;56(5):1263-70 PubMed
Appl Environ Microbiol. 1991 Aug;57(8):2141-6 PubMed
Arch Microbiol. 2005 Jan;183(1):19-26 PubMed
FEMS Microbiol Ecol. 2002 Jun 1;40(3):215-22 PubMed
Bacteriol Rev. 1971 Jun;35(2):171-205 PubMed
Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):11-26 PubMed
Theor Appl Genet. 1995 Sep;91(4):589-97 PubMed
J Bacteriol. 1990 May;172(5):2755-61 PubMed
Arch Microbiol. 1988 May;150(1):61-71 PubMed
Biochem Biophys Res Commun. 1982 Dec 15;109(3):675-82 PubMed
