Antioxidant protection during ageing and senescence in chloroplasts of tobacco with modulated life span
Language English Country England, Great Britain Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
18512255
DOI
10.1002/cbf.1481
Knihovny.cz E-resources
- MeSH
- Antioxidants physiology MeSH
- Time Factors MeSH
- Chloroplasts metabolism MeSH
- Cytokinins biosynthesis MeSH
- Plants, Genetically Modified cytology genetics physiology MeSH
- Plant Leaves metabolism MeSH
- Cellular Senescence physiology MeSH
- Nicotiana cytology genetics metabolism physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Antioxidants MeSH
- Cytokinins MeSH
We studied changes in antioxidant protection during ageing and senescence in chloroplasts of tobacco (Nicotiana tabacum L., cv. Wisconsin) with introduced SAG(12) promoter fused with ipt gene for cytokinin synthesis (transgenic plants with increased levels of cytokinins, SAG) or without it (control). Old leaves of SAG plants as well as their chloroplasts maintained higher physiological parameters compared to controls; accordingly, we concluded that their ageing was diverted due to increased cytokinin content. The chloroplast antioxidant protection did not decrease as well. Although antioxidant protection usually decreased in whole leaves of senescing control plants, ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) activity, which maintained the high redox state of ascorbate, increased in chloroplasts of old control leaves.
References provided by Crossref.org
Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis
