Cytokine mRNA expression in porcine cell lines stimulated by enterotoxigenic Escherichia coli
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18514441
DOI
10.1016/j.vetmic.2008.04.024
PII: S0378-1135(08)00157-0
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Cytokines genetics metabolism MeSH
- Enterotoxigenic Escherichia coli physiology MeSH
- Epithelial Cells metabolism microbiology MeSH
- Ileum physiology MeSH
- Macrophages metabolism microbiology MeSH
- RNA, Messenger genetics metabolism MeSH
- Swine MeSH
- Gene Expression Regulation physiology MeSH
- Intestinal Mucosa cytology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytokines MeSH
- RNA, Messenger MeSH
Enterotoxigenic Escherichia coli (ETEC) is an extracellular bacterium that causes post-weaning diarrhoea (PWD) in piglets with different severity of clinical signs. The pathogenesis of ETEC is ascribed to the effect of enterotoxins. ETEC colonizes ileum and probably can penetrate the epithelium and stimulate macrophages. The aim of study was to examine whether there is any difference in cytokine response in vitro produced by two porcine cell lines, intestinal epithelial cell line (IPI-2I) and macrophage cell line (3D4/31) after stimulation with different serotypes of ETEC associated with different clinical course of PWD in piglets. Three serotypes, O149:K88 (F4), O147:F18 and O8:K88, were used. We observed that all the used serotypes were unable to induce IL-8 and TNF-alpha mRNA expression in IPI-2I cell line as measured by the real-time RT-PCR. In 3D4/31 cell line, we detected differences in cytokine response among the used serotypes. The highest IL-8 and TNF-alpha mRNA expression in 3D4/31 was detected after stimulation with serotype O149:K88 frequently associated with hemorrhagic gastroenteritis.
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