Tobacco cells transformed with the fission yeast Spcdc25 mitotic inducer display growth and morphological characteristics as well as starch and sugar status evocable by cytokinin application
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18550380
DOI
10.1016/j.plaphy.2008.04.017
PII: S0981-9428(08)00054-5
Knihovny.cz E-zdroje
- MeSH
- buněčný cyklus účinky léků genetika fyziologie MeSH
- cytokininy metabolismus farmakologie MeSH
- fungální proteiny genetika fyziologie MeSH
- geneticky modifikované rostliny účinky léků genetika metabolismus MeSH
- kultivované buňky MeSH
- proteiny buněčného cyklu genetika fyziologie MeSH
- ras-GRF1 genetika fyziologie MeSH
- regulace genové exprese u rostlin účinky léků MeSH
- sacharidy analýza MeSH
- Schizosaccharomyces genetika MeSH
- škrob analýza MeSH
- tabák cytologie genetika růst a vývoj MeSH
- transformace genetická MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cytokininy MeSH
- fungální proteiny MeSH
- proteiny buněčného cyklu MeSH
- ras-GRF1 MeSH
- sacharidy MeSH
- škrob MeSH
In plants, the G2/M control of cell cycle remains an elusive issue as doubts persist about activatory dephosphorylation--in other eukaryotes provided by CDC25 phosphatase and serving as a final all-or-nothing mitosis regulator. We report on the effects of tobacco (Nicotiana tabacum L., cv. Samsun) transformation with fission yeast (Schizosaccharomyces pombe) cdc25 (Spcdc25) on cell characteristics. Transformed cell suspension cultures showed higher dry mass accumulation during the exponential phase and clustered more circular cell phenotypes compared to chains of elongated WT cells. Similar cell parameters, as in the transformants, can be induced in WT by cytokinins. Spcdc25 cells, after cytokinin treatment, showed giant cell clusters and growth inhibition. In addition, Spcdc25 expression led to altered carbohydrate status: increased starch and soluble sugars with higher sucrose:hexoses ratio, inducible in WT by cytokinin treatment. Taken together, the Spcdc25 transformation had a cytokinin-like effect on studied characteristics. However, endogenous cytokinin determination revealed markedly lower cytokinin levels in Spcdc25 transformants. This indicates that the cells sense Spcdc25 expression as an increased cytokinin availability, manifested by changed cell morphology, and in consequence decrease endogenous cytokinin levels. Clearly, the results on cell growth and morphology are consistent with the model of G2/M control including cytokinin-regulated activatory dephosphorylation. Nevertheless, no clear link is obvious between Spcdc25 transformation and carbohydrate status and thus the observed cytokinin-like effect on carbohydrate levels poses a problem. Hence, we propose that Spcdc25-induced higher CDK(s) activity at G2/M generates a signal-modifying carbohydrate metabolism to meet high energy and C demands of forthcoming cell division.
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