Room temperature photooxidation of beta-carotene and peripheral chlorophyll in photosystem II reaction centre
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- beta Carotene metabolism MeSH
- Chlorophyll metabolism MeSH
- Ferricyanides metabolism MeSH
- Photosynthesis MeSH
- Photosystem II Protein Complex metabolism MeSH
- Pisum sativum MeSH
- Molybdenum metabolism MeSH
- Oxidation-Reduction MeSH
- Silicon Compounds metabolism MeSH
- Light MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- beta Carotene MeSH
- Chlorophyll MeSH
- Ferricyanides MeSH
- Photosystem II Protein Complex MeSH
- hexacyanoferrate III MeSH Browser
- Molybdenum MeSH
- silicomolybdate MeSH Browser
- Silicon Compounds MeSH
Differential kinetic absorption spectra were measured during actinic illumination of photosystem II reaction centres and core complexes in the presence of electron acceptors silicomolybdate and ferricyanide. The spectra of samples with ferricyanide differ from those with both ferricyanide and silicomolybdate. Near-infrared spectra show temporary beta-carotene and peripheral chlorophyll oxidation during room temperature actinic illumination. Peripheral chlorophyll is photooxidized even after decay of beta-carotene oxidation activity and significant reduction of beta-carotene content in both reaction centres and photosystem II core complexes. Besides, new carotenoid cation is observed after about 1 s of actinic illumination in the reaction centres when silicomolybdate is present. Similar result was observed in PSII core complexes. HPLC analyses of illuminated reaction centres reveal several novel carotenoids, whereas no new carotenoid species were observed in HPLC of illuminated core complexes. Our data support the proposal that pigments of inner antenna are a sink of cations originating in the photosystem II reaction centre.
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