Effects of resveratrol pretreatment on tert-butylhydroperoxide induced hepatocyte toxicity in immobilized perifused hepatocytes: involvement of inducible nitric oxide synthase and hemoxygenase-1
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18812229
DOI
10.1016/j.niox.2008.08.006
PII: S1089-8603(08)00364-9
Knihovny.cz E-resources
- MeSH
- Alanine Transaminase metabolism MeSH
- Analysis of Variance MeSH
- Apoptosis drug effects MeSH
- Cytoprotection MeSH
- Heme Oxygenase-1 metabolism MeSH
- Hepatocytes drug effects metabolism ultrastructure MeSH
- Cells, Immobilized MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Biomarkers, Tumor metabolism MeSH
- Nitric Oxide metabolism MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Rats, Wistar MeSH
- Resveratrol MeSH
- Silymarin pharmacology MeSH
- Stilbenes pharmacology MeSH
- Nitric Oxide Synthase Type II metabolism MeSH
- tert-Butylhydroperoxide toxicity MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alanine Transaminase MeSH
- Heme Oxygenase-1 MeSH
- Biomarkers, Tumor MeSH
- Nitric Oxide MeSH
- Resveratrol MeSH
- Silymarin MeSH
- Stilbenes MeSH
- Nitric Oxide Synthase Type II MeSH
- tert-Butylhydroperoxide MeSH
The aim of this work was to study the effects of resveratrol (RES) as compared to silymarin (SM) pretreatments on tert-butylhydroperoxide (tBH) induced apoptotic/necrotic markers in hepatocytes. Hepatocyte in cultures (48 h) and in perifused immobilized agarose threads (5h) were used as cellular systems. Hepatocyte apoptosis was estimated morphologically using Annexin-V combined with propidium iodide, or toluidine blue staining. Hepatocyte viability and functionality were evaluated by ALT and urea synthesis. Nitric oxide (NO) and carbon monoxide involvements were also examined. Resveratrol and silymarin reduced tBH-induced hepatocyte toxic effects in short term experiments (5h) as measured by a significant reduction in ALT and NO increase produced by tBH. Both inducible nitric oxide synthase (NOS-2) and hemoxygenase-1 (HO-1) gene expression were increased by tBH and reduced by both RES and SM pretreatments. Morphologically, there were ameliorations in both apoptotic and necrotic markers under RES treatment and were similar to biochemical findings. In addition, RES improved hepatocyte stability in both cellular systems. It may be concluded that resveratrol and sylimarin ameliorative effects on tBH hepatocyte toxicity are comparable; involve NOS-2 and HO-1 expression and should be re-evaluated in various in vitro and in vivo experimental conditions.
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