Identification of collagen types in tissues using HPLC-MS/MS
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Calibration MeSH
- Collagen analysis genetics MeSH
- Rats MeSH
- Humans MeSH
- Lod Score MeSH
- Protein Isoforms analysis genetics MeSH
- Amino Acid Sequence MeSH
- Cattle MeSH
- Tandem Mass Spectrometry MeSH
- Tissue Extracts chemistry MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Collagen MeSH
- Protein Isoforms MeSH
- Tissue Extracts MeSH
A method for the determination and quantification of collagen types I-V in rat tissues has been developed. This method is based on collagen fragmentation by cyanogen bromide followed by trypsin digestion. After that, HPLC-MS/MS (HPLC coupled to an IT mass spectrometer) analyses of the resulting peptide mixtures (peptide maps) were performed. Specific peptides for each collagen type were selected. According to online databases, these peptides are present in human, bovine, and rat collagens. As a result, this method can be potentially applied to other species' tissues as well, such as human tissues, and provides a universal and simple method of quantifying collagen types. The applicability of this method for analyzing collagen types was demonstrated on rat tissues (skin, tendon, and aorta).
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