Identification of collagen types in tissues using HPLC-MS/MS
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18837476
DOI
10.1002/jssc.200800351
Knihovny.cz E-zdroje
- MeSH
- kalibrace MeSH
- kolagen analýza genetika MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- Lod skóre MeSH
- protein - isoformy analýza genetika MeSH
- sekvence aminokyselin MeSH
- skot MeSH
- tandemová hmotnostní spektrometrie MeSH
- tkáňové extrakty chemie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kolagen MeSH
- protein - isoformy MeSH
- tkáňové extrakty MeSH
A method for the determination and quantification of collagen types I-V in rat tissues has been developed. This method is based on collagen fragmentation by cyanogen bromide followed by trypsin digestion. After that, HPLC-MS/MS (HPLC coupled to an IT mass spectrometer) analyses of the resulting peptide mixtures (peptide maps) were performed. Specific peptides for each collagen type were selected. According to online databases, these peptides are present in human, bovine, and rat collagens. As a result, this method can be potentially applied to other species' tissues as well, such as human tissues, and provides a universal and simple method of quantifying collagen types. The applicability of this method for analyzing collagen types was demonstrated on rat tissues (skin, tendon, and aorta).
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