A novel and fast simultaneous determination of triamcinolone acetonide (TCA) and salicylic acid (SA) in topical pharmaceutical formulations by sequential injection chromatography (SIC) as an alternative to classical high performance liquid chromatography (HPLC) has been developed. A recently introduced Onyxtrade mark monolithic C18 (50mmx4.6mm, Phenomenex((R))) with 5mm monolithic precolumn were used for the first time for creating sequential injection chromatography system based on a FIAlab((R)) 3000 with a six-port selection valve and 5.0mL syringe pump in study. The mobile phase used was acetonitrile/water (35:65, v/v), pH 3.3 adjusted with acetic acid at flow rate 0.9mLmin(-1). UV detection provided by fibre-optic DAD detector was set up at 240nm. Propylparaben was chosen as suitable internal standard (IS). There is only simple pre-adjustment of the sample of topical solution (dilution with mobile phase) so the analysis is not uselessly elongated. Parameters of the method showed good linearity in wide range, correlation coefficient >0.999; system precision (relative standard deviation, R.S.D.) in the range 0.45-1.95% at three different concentration levels, detection limits (3sigma) 1.00mugmL(-1) (salicylic acid), 0.66mugmL(-1) (triamcinolone acetonide) and 0.33mugmL(-1) (propylparaben) and recovery from the pharmaceutical preparations in the range 97.50-98.94%. The chromatographic resolution between peaks of compounds was more than 4.5 and analysis time was 5.1min under the optimal conditions. The advantages of sequential injection chromatography against classical HPLC are discussed and showing that SIC can be a method of option in many cases.

Department of Analytical Chemistry Faculty of Pharmacy Charles University Heyrovského 1203 Hradec Králové 500 05 Czech Republic

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