Phylogeny of Flabellulidae (Amoebozoa: Leptomyxida) inferred from SSU rDNA sequences of the type strain of Flabellula citata Schaeffer, 1926 and newly isolated strains of marine amoebae
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19175203
Knihovny.cz E-resources
- MeSH
- Amebiasis genetics parasitology veterinary MeSH
- Amoeba classification isolation & purification ultrastructure MeSH
- Phylogeny MeSH
- Ribosome Subunits, Small genetics MeSH
- Molecular Sequence Data MeSH
- Fish Diseases genetics parasitology MeSH
- Polymerase Chain Reaction MeSH
- DNA, Protozoan classification genetics MeSH
- DNA, Ribosomal classification genetics MeSH
- Fishes genetics parasitology MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Sequence Alignment MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Protozoan MeSH
- DNA, Ribosomal MeSH
New strains of non-vannellid flattened amoebae isolated from fish, an invertebrate and the marine environment were studied together with Flabellula citata Schaeffer, 1926 selected by morphology as a reference strain. The study revealed a paucity of features distinguishing individual strains at the generic level, but clearly evidenced mutual phylogenetic relationships within the assemblage of strains as well as their affiliation to the Leptomyxida. In this study, the SSU rDNA dataset of leptomyxids was expanded and a new branching pattern was presented within this lineage of Amoebozoa. Sequences of three newly introduced strains clustered in close relationship with the type strain of F. citata, the type species of the genus. Three strains, including one resembling Flamella sp., were positioned within a sister-group containing Paraflabellula spp. Results of phylogenetic analysis confirmed doubts of previous authors regarding generic assignment of several Rhizanmoeba and Ripidomnyxa strains.
