Extraction of PCR-ready DNA from Staphylococcus aureus bacteriophages using carboxyl functionalized magnetic nonporous microspheres
Language English Country Netherlands Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
19188099
DOI
10.1016/j.jchromb.2009.01.006
PII: S1570-0232(09)00023-3
Knihovny.cz E-resources
- MeSH
- DNA, Viral genetics isolation & purification MeSH
- Solid Phase Extraction methods MeSH
- Genetic Techniques * MeSH
- Magnetics * MeSH
- Microspheres * MeSH
- Polymerase Chain Reaction MeSH
- Staphylococcus Phages genetics isolation & purification MeSH
- Staphylococcus aureus virology MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Viral MeSH
Magnetic microspheres P(HEMA-co-EDMA) were used for PCR-ready phage DNA isolation from lysogenic strains of Staphylococcus aureus, including two new clinical isolates. The conditions of phage particle lysis were optimized. The quality of eluted phage DNA was evaluated by PCR. It was demonstrated that PCR-ready phage DNA can be isolated from small volumes of phage lysates (150 microl) by magnetic microspheres. The reported method is very expeditious without using toxic compounds such as phenol or chloroform. It can be used for phage identification and phage gene detection.
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