Absolute levels of transcripts for mitochondrial uncoupling proteins UCP2, UCP3, UCP4, and UCP5 show different patterns in rat and mice tissues
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- DNA primery genetika MeSH
- druhová specificita MeSH
- iontové kanály metabolismus MeSH
- krysa rodu Rattus MeSH
- membránové transportní proteiny metabolismus MeSH
- messenger RNA metabolismus MeSH
- mitochondriální odpřahující proteiny MeSH
- mitochondriální proteiny metabolismus MeSH
- mozek metabolismus MeSH
- myokard metabolismus MeSH
- myši MeSH
- plíce metabolismus MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- proteiny nervové tkáně metabolismus MeSH
- slezina metabolismus MeSH
- transportní proteiny mitochondriální membrány MeSH
- uncoupling protein 2 MeSH
- uncoupling protein 3 MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- DNA primery MeSH
- iontové kanály MeSH
- membránové transportní proteiny MeSH
- messenger RNA MeSH
- mitochondriální odpřahující proteiny MeSH
- mitochondriální proteiny MeSH
- proteiny nervové tkáně MeSH
- Slc25a14 protein, rat MeSH Prohlížeč
- Slc25a27 protein, rat MeSH Prohlížeč
- transportní proteiny mitochondriální membrány MeSH
- Ucp2 protein, mouse MeSH Prohlížeč
- Ucp2 protein, rat MeSH Prohlížeč
- Ucp3 protein, mouse MeSH Prohlížeč
- Ucp3 protein, rat MeSH Prohlížeč
- uncoupling protein 2 MeSH
- uncoupling protein 3 MeSH
Existing controversies led us to analyze absolute mRNA levels of mitochondrial uncoupling proteins (UCP1-UCP5). Individual UCP isoform mRNA levels varied by up to four orders of magnitude in rat and mouse tissues. UCP2 mRNA content was relatively high (0.4 to 0.8 pg per 10 ng of total mRNA) in rat spleen, rat and mouse lung, and rat heart. Levels of the same order of magnitude were found for UCP3 mRNA in rat and mouse skeletal muscle, for UCP4 and UCP5 mRNA in mouse brain, and for UCP2 and UCP5 mRNA in mouse white adipose tissue. Significant differences in pattern were found for rat vs. mouse tissues, such as the dominance of UCP3/UCP5 vs. UCP2 transcript in mouse heart and vice versa in rat heart; or UCP2 (UCP5) dominance in rat brain contrary to 10-fold higher UCP4 and UCP5 dominance in mouse brain. We predict high antioxidant/antiapoptotic UCP function in tissues with higher UCP mRNA content.
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Mitochondrial Uncoupling Proteins: Subtle Regulators of Cellular Redox Signaling
