Determination of tolterodine and its 5-hydroxymethyl metabolite in human plasma by hydrophilic interaction liquid chromatography-tandem mass spectrometry
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články
PubMed
19269904
DOI
10.1016/j.jchromb.2009.02.036
PII: S1570-0232(09)00135-4
Knihovny.cz E-zdroje
- MeSH
- benzhydrylové sloučeniny krev chemie farmakokinetika MeSH
- dospělí MeSH
- fenylpropanolamin krev chemie farmakokinetika MeSH
- kresoly krev chemie farmakokinetika MeSH
- lidé MeSH
- mladý dospělý MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- tolterodin tartarát MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Názvy látek
- benzhydrylové sloučeniny MeSH
- fenylpropanolamin MeSH
- kresoly MeSH
- tolterodin tartarát MeSH
A rapid and reliable method was developed to quantitate tolterodine and its 5-hydroxymethyl metabolite in human plasma using liquid chromatography-electrospray tandem mass spectrometry. The assay was based on liquid-liquid extraction of the compounds from plasma with tert-butylmethylether and hydrophilic interaction chromatography performed on a silica column (30mmx4.6mm, 3microm particles), the mobile phase consisted of acetonitrile-20mM ammonium acetate (70:30, v/v). Quantification was through positive-ion mode and selected reaction monitoring at m/z 326-->147 for tolterodine, 342-->223 for the 5-hydroxymethyl metabolite and 260-->183 for the internal standard propranolol, respectively. The lower limit of quantitation was 49 and 46pg/ml using 0.5ml of plasma for the parent drug and its metabolite, respectively and linearity was observed up to 30ng/ml. Within-day and between-day precision expressed by relative standard deviation was less than 11% and inaccuracy did not exceed 7% at all levels. The assay was applied to the analysis of samples from a pharmacokinetic study.
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