Photodynamic properties of ZnTPPS(4), ClAlPcS(2) and ALA in human melanoma G361 cells
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19720133
DOI
10.1016/j.tiv.2009.08.015
PII: S0887-2333(09)00239-2
Knihovny.cz E-resources
- MeSH
- Coloring Agents MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Fluorescent Dyes MeSH
- Microscopy, Fluorescence MeSH
- Photochemotherapy * MeSH
- Photosensitizing Agents pharmacology MeSH
- DNA Fragmentation drug effects radiation effects MeSH
- Indoles pharmacology MeSH
- Aminolevulinic Acid pharmacology MeSH
- Humans MeSH
- Melanoma, Experimental therapy MeSH
- Membrane Potentials drug effects MeSH
- Metalloporphyrins pharmacology MeSH
- Mitochondrial Membranes drug effects MeSH
- Cell Line, Tumor MeSH
- Organometallic Compounds pharmacology MeSH
- Propidium MeSH
- Reactive Oxygen Species metabolism MeSH
- Rhodamine 123 MeSH
- Light MeSH
- Tetrazolium Salts MeSH
- Thiazoles MeSH
- Dose-Response Relationship, Radiation MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- aluminum phthalocyanine disulfonate MeSH Browser
- Coloring Agents MeSH
- Fluorescent Dyes MeSH
- Photosensitizing Agents MeSH
- Indoles MeSH
- Aminolevulinic Acid MeSH
- Metalloporphyrins MeSH
- Organometallic Compounds MeSH
- Propidium MeSH
- Reactive Oxygen Species MeSH
- Rhodamine 123 MeSH
- Tetrazolium Salts MeSH
- Thiazoles MeSH
- thiazolyl blue MeSH Browser
- zinc-tetrakis(p-sulfonatophenyl)porphyrin MeSH Browser
Photodynamic therapy (PDT) has been approved as proper and effective kind of treatment for certain types of cancer and non-malignant diseases. We tested photodynamic effects on G361 human melanoma cells sensitized by zinc-5,10,15,20-tetrakis(4-sulphonatophenyl) porphyrine (ZnTPPS(4)), chloraluminium phtalocyanine disulfonate (ClAlPcS(2)) and 5-aminolevulinic acid (ALA). In particular, we examined the PDT efficiency depending on applied light dose (0.8; 1.7; 3.3; 6.6; 13.2; 26.4Jcm(-2)). The DNA gel electrophoresis, methylthiazol tetrazolium bromide (MTT) viability test, fluorescent microscopy using calcein AM and propidium iodide (PI) staining, and rhodamine 123 mitochondrial membrane potential assay were performed to detect and evaluate the cell death process. We also measured the time course of reactive oxygen species (ROS) production and its dependence on sensitizer concentration within continuously irradiated sensitized cells. In conclusion, these results demonstrate most significant phototoxic effect of ClAlPcS(2)-PDT in spite of significantly higher ROS production induced by ZnTPPS(4)-PDT on G361 cells. On the other hand, ALA-PDT has a minimal photoeffect and induces negligible ROS formation in G361 cells at the conditions described below.
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