Molecular weight and polydispersity of calf-thymus DNA: static light-scattering and size-exclusion chromatography with dual detection
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
19817429
DOI
10.1021/bm900768j
Knihovny.cz E-resources
- MeSH
- DNA analysis chemistry MeSH
- Chromatography, Gel * methods MeSH
- Molecular Weight MeSH
- Scattering, Radiation MeSH
- Cattle MeSH
- Light * MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- calf thymus DNA MeSH Browser
- DNA MeSH
Plausible calf-thymus DNA molecular weight distribution can be obtained by size-exclusion chromatography with dual low-angle light scattering/refractometric detection at sufficiently low flow rate. The distribution extends over three decades of molecular weight and is characterized by weight average molecular weight M(w) = 8418000 and polydispersity index M(w)/M(n) = 5.2. After strongly scattering impurities had been removed from the sample using adsorption properties of the 3 mum mixed-cellulose-ester filter membranes, static light-scattering measurement in flow injection mode was feasible and gave M(w) = 8580000, corroborating the veracity of SEC results.
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