Cell-free plasma DNA during Hemodialysis
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19839825
DOI
10.1080/08860220902963673
PII: 10.1080/08860220902963673
Knihovny.cz E-resources
- MeSH
- Cell-Free System MeSH
- Biomarkers blood MeSH
- C-Reactive Protein analysis MeSH
- Time Factors MeSH
- Kidney Failure, Chronic blood mortality therapy MeSH
- Renal Dialysis methods mortality MeSH
- DNA blood MeSH
- Adult MeSH
- Risk Assessment MeSH
- Cohort Studies MeSH
- Middle Aged MeSH
- Humans MeSH
- Membranes, Artificial MeSH
- Survival Rate MeSH
- Plasma Cells MeSH
- Leukocyte Count MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Probability MeSH
- Predictive Value of Tests MeSH
- Prognosis MeSH
- Reference Values MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- C-Reactive Protein MeSH
- DNA MeSH
- Membranes, Artificial MeSH
PURPOSE: Cell-free plasma DNA (cfDNA) levels originating predominantly from apoptotic leukocytes were found to rise during hemodialysis (HD) session, and as such are considered a marker of HD membrane biocompatibility. The purpose of our study was to determine the changes of cfDNA during two consecutive high-flux polysulphone HD sessions after a long (HD-L) and short (HD-S) interdialytic interval. METHODS: A total of 17 HD patients were examined. Prior to HD and at 30 and 240 min, cfDNA (using real-time PCR) and leukocyte count were determined. RESULTS: No significant difference was found when comparing pre-HD-S with pre-HD-L cfDNA [4893 (1090-28804) vs. 4589 (691-73796) genomic equivalent/mL]. A significant increase in cfDNA at 240 min was seen in HD-L (p < 0.05) but not in HD-S. Leukocyte count correlated with cfDNA levels before HD-S (r = 0.8; p < 0.01); however, no other correlation was seen between routinely measured biochemical markers and pre-HD cfDNA levels or cfDNA changes during HD. The increase in plasma cfDNA during HD did not correlate with dialysis duration, its efficacy, or ultrafiltration. An association between magnitude of diuresis and cfDNA levels in HD-S was found (r = 0.58; p < 0.05). CONCLUSIONS: The behavior of cfDNA during HD after long and short interdialytic interval is inconsistent and cannot be explained by changes in laboratory and clinical parameters. The observed relationship of plasma cfDNA levels with diuresis deserves further investigation.
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