Epidemiology and characterization of Staphylococcus epidermidis isolates from humans, raw bovine milk and a dairy plant
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19845994
DOI
10.1017/s0950268809991002
PII: S0950268809991002
Knihovny.cz E-resources
- MeSH
- Biofilms growth & development MeSH
- DNA, Bacterial genetics MeSH
- DNA Fingerprinting MeSH
- Phenotype MeSH
- Genotype MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Environmental Microbiology * MeSH
- Milk microbiology MeSH
- Molecular Epidemiology MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Cluster Analysis MeSH
- Cattle MeSH
- Staphylococcus epidermidis classification genetics isolation & purification physiology MeSH
- Bacterial Typing Techniques * MeSH
- Agriculture * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
Geographically related Staphylococcus epidermidis isolates from human patients (n=30), dairy farms (farmers and individual raw milk from cattle, n=36) and a dairy plant (n=55) were examined for epidemiological relatedness by pulsed-field gel electrophoresis and, using in vitro methods, for the ability to produce biofilm and antimicrobial resistance. Methicillin-resistant isolates (MRSE) were also identified and characterized. Isolates from farmers and dairy cattle were found to be genetically related, while isolates from human patients were highly diverse. Some dairy plant isolates (18.2%) were closely related to those from dairy farms. Biofilm production and resistance to antimicrobial agents were most typical for isolates from human patients, of which 76.7% were MRSE. Methicillin resistance was also widespread in farm-related isolates (61.1%). This study indicates the possible transmission of S. epidermidis between cattle and farmers. Dairy products were not proven to be an important source of either human infections or methicillin-resistant strains.
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