Factors for bile tolerance in Lactococcus lactis: analysis by using plasmid variants
Language English Country United States Media print-electronic
Document type Journal Article
- MeSH
- Models, Biological MeSH
- Lactococcus lactis chemistry drug effects genetics physiology MeSH
- Fatty Acids chemistry metabolism MeSH
- Plasmids genetics metabolism MeSH
- Bile MeSH
- Bile Acids and Salts pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fatty Acids MeSH
- Bile Acids and Salts MeSH
The factors of bile tolerance (as one among the fundamental characteristics of probiotic bacteria) were determined in lactococci by using plasmid variants. Bile tolerance of Lactococcus lactis wild-type (WT) strains 527 and N7 (determined by viability counts on bile-containing agar) was equivalent to the corresponding plasmid-free derivatives. In contrast, L. lactis WT strain DRC1 had lower bile tolerance than its plasmid-free derivative DRC1021. Plasmid pDR1-1B, extracted from strain DRC1, was introduced into strain DRC1021 by co-transformation with the vector plasmid pGKV21 as an indicator. Strain DRC121 (DRC1021 harboring pGKV21) had good bile tolerance as did strain DRC1021, while strain DRC13 (DRC1021 harboring both pDR1-1B and pGKV21) did not. Fatty acid (FA) composition was different between strains DRC121 and DRC13. The plasmid pDR1-1B or plasmid profile and FA composition are key factors for bile tolerance of strain DRC1, and therefore changing the plasmid profile might be a way of modulating bile tolerance in lactococci.
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Variations in bile tolerance among Lactococcus lactis strains derived from different sources
