Time-dependent changes in concentration of two clinically used acetylcholinesterase reactivators (HI-6 and obidoxime) in rat plasma determined by HPLC techniques after in vivo administration
Language English Country Switzerland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20065589
DOI
10.2116/analsci.26.63
PII: JST.JSTAGE/analsci/26.63
Knihovny.cz E-resources
- MeSH
- Indicators and Reagents MeSH
- Injections, Intramuscular MeSH
- Calibration MeSH
- Blood Proteins chemistry MeSH
- Rats MeSH
- Trichloroacetic Acid chemistry MeSH
- Obidoxime Chloride blood pharmacokinetics MeSH
- Oximes blood pharmacokinetics MeSH
- Rats, Wistar MeSH
- Pyridinium Compounds blood pharmacokinetics MeSH
- Cholinesterase Reactivators blood pharmacokinetics MeSH
- Reproducibility of Results MeSH
- Spectrophotometry, Ultraviolet MeSH
- Chromatography, High Pressure Liquid MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- asoxime chloride MeSH Browser
- Indicators and Reagents MeSH
- Blood Proteins MeSH
- Trichloroacetic Acid MeSH
- Obidoxime Chloride MeSH
- Oximes MeSH
- Pyridinium Compounds MeSH
- Cholinesterase Reactivators MeSH
A simple and reliable HPLC method for determination of rat plasma levels of clinically used acetylcholinesterase (AChE) reactivators (HI-6 and obidoxime) is presented in our study. Separation was carried out by HPLC using an octadecyl silica stationary phase and a mobile phase consisting of 24% acetonitrile and containing 5 mM sodium octanesulfonate and 5 mM tetramethylammonium chloride (pH 2.3). Following intramuscular administration of equimolar doses of both oximes (22.23 mg/kg), the maximum of HI-6 concentration in rat plasma was reached in about 20 min giving 15.26 +/- 1.71 microg/mL. The distribution of obidoxime was fast; the single maximum 23.62 +/- 3.563 microg/mL was recorded at about 10 min. HPLC with UV detection presented in our study is a general method which could be applied for quick measurements of bisquaternary AChE reactivators in rat plasma.
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