The effect of interferon-gamma and lipopolysaccharide on the growth of Francisella tularensis LVS in murine macrophage-like cell line J774
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Line MeSH
- Francisella tularensis growth & development MeSH
- Interferon-gamma pharmacology MeSH
- Lipopolysaccharides pharmacology MeSH
- Macrophages drug effects microbiology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Recombinant Proteins MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Interferon-gamma MeSH
- Lipopolysaccharides MeSH
- Recombinant Proteins MeSH
BACKGROUND: Francisella tularensis, a causative agent of human tularemia, displaying the ability to proliferate inside the human cells. AIMS: To evaluate the growth potential of F. tularensis LVS strain in macrophage-like cell line J774 modulated by recombinant interferon gamma and E. coli derived lipopolysaccharide. RESULTS: Stimulation of J774 cells either by interferon-gamma or lipopolysaccharide alone, or especially in combination before infection F. tularensis, revealed protective effects. Higher concentrations of stimulating agents were needed to inhibit ongoing F. tularensis infection. CONCLUSIONS: Stimulation of J774 cell line by combination of interferon-gamma with lipopolysaccharide inhibits the intracellular growth of F. tularensis.
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