Sexual dimorphism in stress-induced changes in adrenergic and muscarinic receptor densities in the lung of wild type and corticotropin-releasing hormone-knockout mice
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Receptors, Adrenergic, alpha-1 metabolism MeSH
- Receptors, Adrenergic, beta metabolism MeSH
- Restraint, Physical MeSH
- Stress, Physiological physiology MeSH
- Corticotropin-Releasing Hormone genetics metabolism MeSH
- Mice, Knockout MeSH
- Mice MeSH
- Lung metabolism MeSH
- Sex Characteristics * MeSH
- Receptors, Muscarinic metabolism MeSH
- Sex Factors MeSH
- Pituitary-Adrenal System metabolism MeSH
- Hypothalamo-Hypophyseal System metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Receptors, Adrenergic, alpha-1 MeSH
- Receptors, Adrenergic, beta MeSH
- Corticotropin-Releasing Hormone MeSH
- Receptors, Muscarinic MeSH
We tested the hypothesis that single and repeated immobilization stress affect densities of alpha(1)-adrenoceptor (alpha(1)-AR) and beta-AR subtypes, muscarinic receptors (MR), adenylyl cyclase activity (AC) and phospholipase C activity (PLC) in lungs of male and female wild type (WT) and corticotropin-releasing hormone gene (CRH-knockout (KO)) disrupted mice. We found sex differences in the basal levels of alpha(1)-AR subtypes (females had 2-3 times higher density of receptors than males) and MR (males had twice the density found in females). In marked contrast, beta-AR subtype densities did not differ between sexes. CRH gene disruption decreased all three studied receptors in intact mice (to 20-50% of WT) in both sexes (except beta(1)-AR in females). Stress induced sexually dimorphic responses, while all alpha(1)-AR subtypes decreased in females (to 30% of control approximately), only alpha(1A)-AR level diminished (about 50%) in males. beta(1)-AR decreased in males (to about 40%) but remained stable in females. beta(2)-AR diminished in females (to about 20-60%) and also in males (to about 30-60%). MR decreased in both sexes (approximately to 50%). AC activity diminished in males (to < 50%) while PLC activity was not changed. In CRH-KO mice, the stress response was severely diminished. Paradoxically, the receptor response to stress was less affected by CRH-KO in males than in females. AC activity did not change in CRH-KO mice. In conclusion, in mice the stress reaction is sexually dimorphic and an intact hypothalamo-pituitary-adrenocortical system is required for the normal reaction of pulmonary adrenergic and MR to stress.
References provided by Crossref.org
Acute restraint stress modifies the heart rate biorhythm in the poststress period
Lack of CRH Affects the Behavior but Does Not Affect the Formation of Short-Term Memory
