Strains of mice in which certain GENES of their GENOMES have been disrupted, or knocked-out. To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.

Annotation

NIM no qualif when exper animal; do not forget also to check MICE

DUI

D018345 MeSH Browser

CUI

M0027579

Previous indexing

Mice (1992-1993)

History note

94

Public note

94