Cloning a transgene for transgenic RNAi in mouse oocytes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
20147029
DOI
10.1101/pdb.prot5134
PII: 2009/1/pdb.prot5134
Knihovny.cz E-zdroje
- MeSH
- Bacteria metabolismus MeSH
- klonování DNA * MeSH
- molekulární sekvence - údaje MeSH
- myši transgenní MeSH
- myši MeSH
- oocyty cytologie MeSH
- plazmidy metabolismus MeSH
- repetitivní sekvence nukleových kyselin MeSH
- RNA interference * MeSH
- sekvence nukleotidů MeSH
- transgeny * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing for many model systems. The protocol presented here describes the cloning of a plasmid construct for use in transgenic RNAi experiments in mouse oocytes. The protocol is intended for production of a transgene by cloning an inverted repeat (IR) into the Zp3 transgenic cassette. The procedure begins with the selection of sequences and formulation of the cloning strategy. Subsequently, the IR is cloned, inserted into the transgenic cassette, and characterized by sequencing. Finally, the transgene is released from the cassette, purified, and provided to the transgenic facility.
Citace poskytuje Crossref.org
Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes
