Effects of exposure to sublethal propiconazole on intestine-related biochemical responses in rainbow trout, Oncorhynchus mykiss
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
20193671
DOI
10.1016/j.cbi.2010.02.040
PII: S0009-2797(10)00108-0
Knihovny.cz E-zdroje
- MeSH
- amylasy metabolismus MeSH
- antioxidancia metabolismus MeSH
- biologické markery analýza MeSH
- chemické látky znečišťující vodu farmakologie MeSH
- karbonylace proteinů účinky léků MeSH
- Oncorhynchus mykiss metabolismus MeSH
- oxidační stres MeSH
- peroxidace lipidů účinky léků MeSH
- proteasy metabolismus MeSH
- střeva účinky léků MeSH
- střevní sliznice metabolismus MeSH
- triazoly farmakologie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- amylasy MeSH
- antioxidancia MeSH
- biologické markery MeSH
- chemické látky znečišťující vodu MeSH
- propiconazole MeSH Prohlížeč
- proteasy MeSH
- triazoly MeSH
The effect of long-term (30 days) exposure to PCZ (0.2, 50, and 500 microg l(-1)) on intestine-related biochemical markers in rainbow trout was investigated. Multiple biomarkers were measured, including digestive enzymes (proteolytic enzymes and amylase), antioxidant responses (TBARS, CP, SOD, CAT, GR and GPx) and energy metabolic parameters (RNA/DNA ratio, Na(+)-K(+)-ATPase). Exposure to 500 microg l(-1) PCZ led to significantly inhibited (p<0.01) proteolytic enzyme and amylase activity. Activities of the antioxidant enzymes SOD, CAT, and GPx gradually increased at lower PCZ concentrations (0.2 and 50 microg l(-1)). At the highest concentration (500 microg l(-1)), oxidative stress was apparent as significant higher (p<0.05) lipid peroxidation and protein carbonyls, associated with an inhibition of antioxidant enzymes activity. Moreover, energy metabolic parameters (RNA/DNA ratio, Na(+)-K(+)-ATPase) were significantly inhibited (p<0.01) in the intestines of fish exposed to 500 microg l(-1) PCZ, compared with controls. We suggest that long-term exposure to PCZ could result in several responses in intestine-related biochemical markers, which potentially could be used as indicators for monitoring residual PCZ present in the aquatic environment.
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