Effects of exposure to sublethal propiconazole on intestine-related biochemical responses in rainbow trout, Oncorhynchus mykiss
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20193671
DOI
10.1016/j.cbi.2010.02.040
PII: S0009-2797(10)00108-0
Knihovny.cz E-resources
- MeSH
- Amylases metabolism MeSH
- Antioxidants metabolism MeSH
- Biomarkers analysis MeSH
- Water Pollutants, Chemical pharmacology MeSH
- Protein Carbonylation drug effects MeSH
- Oncorhynchus mykiss metabolism MeSH
- Oxidative Stress MeSH
- Lipid Peroxidation drug effects MeSH
- Peptide Hydrolases metabolism MeSH
- Intestines drug effects MeSH
- Intestinal Mucosa metabolism MeSH
- Triazoles pharmacology MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amylases MeSH
- Antioxidants MeSH
- Biomarkers MeSH
- Water Pollutants, Chemical MeSH
- propiconazole MeSH Browser
- Peptide Hydrolases MeSH
- Triazoles MeSH
The effect of long-term (30 days) exposure to PCZ (0.2, 50, and 500 microg l(-1)) on intestine-related biochemical markers in rainbow trout was investigated. Multiple biomarkers were measured, including digestive enzymes (proteolytic enzymes and amylase), antioxidant responses (TBARS, CP, SOD, CAT, GR and GPx) and energy metabolic parameters (RNA/DNA ratio, Na(+)-K(+)-ATPase). Exposure to 500 microg l(-1) PCZ led to significantly inhibited (p<0.01) proteolytic enzyme and amylase activity. Activities of the antioxidant enzymes SOD, CAT, and GPx gradually increased at lower PCZ concentrations (0.2 and 50 microg l(-1)). At the highest concentration (500 microg l(-1)), oxidative stress was apparent as significant higher (p<0.05) lipid peroxidation and protein carbonyls, associated with an inhibition of antioxidant enzymes activity. Moreover, energy metabolic parameters (RNA/DNA ratio, Na(+)-K(+)-ATPase) were significantly inhibited (p<0.01) in the intestines of fish exposed to 500 microg l(-1) PCZ, compared with controls. We suggest that long-term exposure to PCZ could result in several responses in intestine-related biochemical markers, which potentially could be used as indicators for monitoring residual PCZ present in the aquatic environment.
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