Neoplastic progression of the human breast cancer cell line G3S1 is associated with elevation of cytoskeletal dynamics and upregulation of MT1-MMP
Jazyk angličtina Země Řecko Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
20198326
DOI
10.3892/ijo_00000560
Knihovny.cz E-zdroje
- MeSH
- aktiny metabolismus MeSH
- aprotinin farmakologie MeSH
- cytoskelet účinky léků enzymologie patologie MeSH
- dipeptidy farmakologie MeSH
- inhibitory matrixových metaloproteinas MeSH
- inhibitory proteas farmakologie MeSH
- invazivní růst nádoru MeSH
- leucin analogy a deriváty farmakologie MeSH
- lidé MeSH
- matrixová metaloproteinasa 14 metabolismus MeSH
- metaloproteinasy secernované do matrix metabolismus MeSH
- mořské toxiny farmakologie MeSH
- nádorové buněčné linie MeSH
- nádorové kmenové buňky enzymologie patologie MeSH
- nádory prsu enzymologie patologie MeSH
- pohyb buněk * účinky léků MeSH
- progrese nemoci MeSH
- pseudopodia enzymologie MeSH
- upregulace MeSH
- želatina metabolismus MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- aktiny MeSH
- aprotinin MeSH
- dipeptidy MeSH
- E 64 MeSH Prohlížeč
- inhibitory matrixových metaloproteinas MeSH
- inhibitory proteas MeSH
- leucin MeSH
- matrixová metaloproteinasa 14 MeSH
- metaloproteinasy secernované do matrix MeSH
- MMP14 protein, human MeSH Prohlížeč
- mořské toxiny MeSH
- N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide MeSH Prohlížeč
- želatina MeSH
The newly established breast cancer cell line G3S1, derived from EM-G3 breast cancer progenitors, was analyzed for functional changes related to neoplastic progression manifested by elevated invasiveness and enhanced capability to degrade gelatin. Degradation of gelatin and invasiveness of G3S1 cells was found to be dependent on the activity of matrix proteinases and actin cytoskeletal dynamics. Therefore, the expression and activity of these proteases was compared in G3S1 and EM-G3 cells. Despite enhanced capability of G3S1 cells to degrade gelatin, these cells exhibited lower levels of secreted extracellular matrix degrading proteases than parental EM-G3 cells. However, the expression of membrane-bound MT1-MMP was strongly elevated in G3S1 cells. While the degradation of gelatin was associated with invadopodia-like structures in both EM-G3 and G3S1 cells, the cytoskeletal remodeling dynamics was greatly elevated in G3S1 cells, suggesting that upregulation of MT1-MMP, together with elevation of cytoskeletal remodeling dynamics can effectively cause elevated invasiveness and enhanced matrix degrading capability in G3S1 cells.
Department of Cell Biology Faculty of Science Charles University Prague 128 43 Prague Czech Republic
Citace poskytuje Crossref.org
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